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實(shí)驗(yàn)常用人肝癌細(xì)胞株(HepG2/Hep3B,HuH-7,MHCC97H,PLC/PRF/5)怎么選?
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CYP4F2抗原;CYP4F2重組蛋白多種屬 該基因編碼細(xì)胞色素P450超家族的一員。細(xì)胞色素P450蛋白是單加氧酶,催化藥物代謝和膽固醇、類固醇和其他脂質(zhì)合成中的許多反應(yīng)。這種蛋白質(zhì)定位于內(nèi)質(zhì)網(wǎng)。這種酶開始滅活和降解白三烯B4的過(guò)程,白三烯是一種強(qiáng)效的炎癥介質(zhì)。該基因是19號(hào)染色體上細(xì)胞色素P450基因簇的一部分。
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品牌 | 信裕生物 | CAS | 詳見(jiàn)說(shuō)明書 |
---|---|---|---|
分子式 | 詳見(jiàn)說(shuō)明書 | 純度 | > 95% |
分子量 | 32.7kDa | 貨號(hào) | XY91399RPHu |
規(guī)格 | 50µg/100µg | 供貨周期 | 一周 |
主要用途 | SDS-PAGE; WB | 應(yīng)用領(lǐng)域 | 生物產(chǎn)業(yè) |
Recombinant Cytochrome P450 4F2 (CYP4F2)
細(xì)胞色素P450家族成員4F2(CYP4F2)重組蛋白
[ PROPERTIES ]
Source:Prokaryotic expression
Host: E.coli
Residues: Met1~Asp252
Tags:N-terminal His Tag
Subcellular Location: Chromosome
Purity: > 95%
Traits:Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Original Concentration:200µg/mL
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:7.7
Predicted Molecular Mass: 32.7kDa
Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8oC for one month.
Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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